NEETZoologyBiotechnology and Its Applications
What was the role of Eli Lilly in the production of insulin using rDNA techniques?
Options
- AIt prepared two DNA sequences corresponding to A and B chains of human insulin.
- BIt introduced the A and B chains of insulin in plasmids of E. coli.
- CIt combined the chains A and B to form human insulin.
- DIt extracted the chains A and B
Correct answer
A. It prepared two DNA sequences corresponding to A and B chains of human insulin.
Step-by-step solution
In mammals, including humans, insulin is synthesized as a pro-hormone (like a pro-enzyme, the pro-hormone also needs to be processed before it becomes a fully mature and functional hormone) which contains an extra stretch called the C peptide. This C peptide is not present in the mature insulin and is removed during maturation into insulin.The main challenge for production of insulin using rDNA techniques was getting insulin assembled into a mature form. In 1983, Eli Lilly an American company prepared two DNA seque