NEETZoologyBiotechnology and Its Applications
What is the function of disulphide bridges in insulin?
Options
- ATo form human insulin
- BTo mature insulin
- CTo remove the C peptide
- DTo link two short polypeptide chains together
Correct answer
D. To link two short polypeptide chains together
Step-by-step solution
In mammals, including humans, insulin is synthesized as a pro-hormone (like a pro-enzyme, the pro-hormone also needs to be processed before it becomes a fully mature and functional hormone) which contains an extra stretch called the C peptide. This C peptide is not present in the mature insulin and is removed during maturation into insulin.The main challenge for production of insulin using rDNA techniques was getting insulin assembled into a mature form. In 1983, Eli Lilly an American company prepared two DNA seque