NEETZoologyBiotechnology - Principles and Processes
What was the basis upon which the discipline of biotechnology was founded?
Options
- ACombining Stanley Cohen's process with Herbert Boyer's DNA splicing
- BThe discovery of the capability of cutting DNA strands in a particular fashion
- CThe capability of inserting DNA in animal cells
- DThe discovery of 'sticky ends' on DNA strands
Correct answer
A. Combining Stanley Cohen's process with Herbert Boyer's DNA splicing
Step-by-step solution
Cohen had been studying small ringlets of DNA called plasmids and which float about freely in the cytoplasm of certain bacterial cells and replicate independently from the coding strand of DNA. Cohen had developed a method of removing these plasmids from the cell and then reinserting them in other cells. Combining this process with that of DNA splicing enabled Boyer and Cohen to recombine segments of DNA in desired configurations and insert the DNA in bacterial cells, which could then act as manufacturing plants fo