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What is the function of restriction endonucleases in genetic engineering?

Options

  1. ATo form recombinant molecules of DNA
  2. BTo break DNA into single stranded portions
  3. CTo join DNA fragments together
  4. DNone of the above

Correct answer

A. To form recombinant molecules of DNA

Step-by-step solution

Restriction enzymes cut the strand of DNA a little away from the center of the palindrome sites, but between the same two bases on the opposite strands . This leaves single stranded portions at the ends. There are overhanging stretches called sticky ends on each strand. These are named so because they form hydrogen bonds with their complementary cut counterparts. This stickiness of the ends facilitates the action of the enzyme DNA ligase. Restriction endonucleases are used in genetic engineering to form ‘recombinan

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