NEETZoologyBiotechnology - Principles and Processes
How do restriction endonucleases cut the strand of DNA?
Options
- ABy cutting the strand of DNA in the middle of the palindrome site
- BBy cutting the strand of DNA a little away from the centre of the palindrome site
- CBy cutting the strand of DNA at the centre of the palindrome site
- DBy cutting the strand of DNA randomly
Correct answer
B. By cutting the strand of DNA a little away from the centre of the palindrome site
Step-by-step solution
: Restriction enzymes cut the strand of DNA a little away from the center of the palindrome sites, but between the same two bases on the opposite strands. This leaves single stranded portions at the ends . There are overhanging stretches called sticky ends on each strand. These are named so because they form hydrogen bonds with their complementary cut counterparts. This stickiness of the ends facilitates the action of the enzyme DNA ligase. Restriction endonucleases are used in genetic engineering to form ‘recombin