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NEET
Exam
NEET
Subject
Zoology
Chapter
Biotechnology - Principles and Processes

NEET · Zoology · Biotechnology - Principles and Processes

What happens when cut by the same restriction enzyme?

What happens when cut by the same restriction enzyme?

Options

  1. AThe resultant DNA fragments have different sticky ends
  2. BThe resultant DNA fragments have the same kind of sticky ends
  3. CThe resultant DNA fragments do not have any sticky ends
  4. DNone of the above

Correct answer

B. The resultant DNA fragments have the same kind of sticky ends

Step-by-step solution

Restriction enzymes cut the strand of DNA a little away from the center of the palindrome sites, but between the same two bases on the opposite strands. This leaves single stranded portions at the ends. There are overhanging stretches called sticky ends on each strand. These are named so because they form hydrogen bonds with their complementary cut counterparts. This stickiness of the ends facilitates the action of the enzyme DNA ligase. Restriction endonucleases are used in genetic engineering to form ‘recombinant’ molecules of DNA, which are composed of DNA from different sources/genomes. When cut by the same restriction enzyme, the resultant DNA fragments have the same kind of ‘sticky-ends’ and, these can be joined together (end-to-end) using DNA ligases

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