NEETZoologyBiotechnology - Principles and Processes
What happens when cut by the same restriction enzyme?
Options
- AThe resultant DNA fragments have different sticky ends
- BThe resultant DNA fragments have the same kind of sticky ends
- CThe resultant DNA fragments do not have any sticky ends
- DNone of the above
Correct answer
B. The resultant DNA fragments have the same kind of sticky ends
Step-by-step solution
Restriction enzymes cut the strand of DNA a little away from the center of the palindrome sites, but between the same two bases on the opposite strands. This leaves single stranded portions at the ends. There are overhanging stretches called sticky ends on each strand. These are named so because they form hydrogen bonds with their complementary cut counterparts. This stickiness of the ends facilitates the action of the enzyme DNA ligase. Restriction endonucleases are used in genetic engineering to form ‘recombinant