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.What is the role of DNA ligase in genetic engineering?

Options

  1. ATo join DNA fragments together
  2. BTo cut DNA into single stranded portions
  3. CTo form recombinant molecules of DNA
  4. DNone of the above

Correct answer

A. To join DNA fragments together

Step-by-step solution

: Restriction enzymes cut the strand of DNA a little away from the center of the palindrome sites, but between the same two bases on the opposite strands. This leaves single stranded portions at the ends. There are overhanging stretches called sticky ends on each strand. These are named so because they form hydrogen bonds with their complementary cut counterparts. This stickiness of the ends facilitates the action of the enzyme DNA ligase. Restriction endonucleases are used in genetic engineering to form ‘recombina

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