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NEET
Exam
NEET
Subject
Zoology
Chapter
Biotechnology - Principles and Processes

NEET · Zoology · Biotechnology - Principles and Processes

What is the function of restriction endonucleases?

What is the function of restriction endonucleases?

Options

  1. ATo separate and isolate DNA fragments
  2. BTo create recombinant vector molecule
  3. CTo form hydrogen bonds
  4. DTo cut DNA into fragments

Correct answer

D. To cut DNA into fragments

Step-by-step solution

Restriction enzymes cut the strand of DNA a little away from the centre of the palindrome sites, but between the same two bases on the opposite strands. This leaves single stranded portions at the ends. There are overhanging stretches called sticky ends on each strand. These are named so because they form hydrogen bonds with their complementary cut counterparts. This stickiness of the ends facilitates the action of the enzyme DNA ligase.

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