NEETZoologyBiotechnology - Principles and Processes
What is the result of cutting DNA by restriction endonucleases?
Options
- AHydrogen bonds
- BRecombinant vector molecule
- CDNA fragments
- DAgarose gel
Correct answer
C. DNA fragments
Step-by-step solution
Restriction enzymes cut the strand of DNA a little away from the center of the palindrome sites, but between the same two bases on the opposite strands. This leaves single stranded portions at the ends. There are overhanging stretches called sticky ends on each strand. These are named so because they form hydrogen bonds with their complementary cut counterparts. This stickiness of the ends facilitates the action of the enzyme DNA ligase.