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NEET
Exam
NEET
Subject
Zoology
Chapter
Biotechnology - Principles and Processes

NEET · Zoology · Biotechnology - Principles and Processes

What is the purpose of ethidium bromide staining?

What is the purpose of ethidium bromide staining?

Options

  1. ATo extract the DNA fragments from agarose gel
  2. BTo join the DNA fragments with cloning vectors
  3. CTo visualize the separated DNA fragments
  4. DTo purify the DNA fragments

Correct answer

C. To visualize the separated DNA fragments

Step-by-step solution

The separated DNA fragments can be visualized only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation (you cannot see pure DNA fragments in the visible light and without staining). You can see bright orange coloured bands of DNA in a ethidium bromide stained gel exposed to UV light. The separated bands of DNA are cut out from the agarose gel and extracted from the gel piece. This step is known as elution. The DNA fragments purified in this way are used in constructing recombinant DNA by joining them with cloning vectors.

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