NEETZoologyBiotechnology - Principles and Processes
What is the matrix most commonly used in gel electrophoresis?
Options
- AEthidium bromide
- BDNA ligase
- CAgarose
- DSticky ends
Correct answer
C. Agarose
Step-by-step solution
The cutting of DNA by restriction endonucleases results in the fragments of DNA. These fragments can be separated by a technique known as gel electrophoresis. Since >DNA fragments are negatively charged molecules they can be separated by forcing them to move towards the anode under an electric field through a medium/matrix. Nowadays the most commonly used matrix is agarose which is a natural polymer extracted from sea weeds. The DNA fragments separate (resolve) according to their size through sieving effect provide