NEET
- Exam
- NEET
- Subject
- Zoology
- Chapter
- Biotechnology - Principles and Processes
NEET · Zoology · Biotechnology - Principles and Processes
What is the importance of having a selectable marker in a vector?
What is the importance of having a selectable marker in a vector?
Options
- ATo identify and eliminate nontransformants
- BTo support high copy number
- CTo start replication
- DTo permit the growth of transformants
Correct answer
D. To permit the growth of transformants
Step-by-step solution
In addition to ‘ori’, the vector requires a selectable marker, which helps in identifying and eliminating nontransformants and selectively permitting the growth of the >transformants. Transformation is a procedure through which a piece of DNA is introduced in a host bacterium (you will study the process in subsequent section). Normally, the genes encoding >resistance to antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin, etc., are considered useful selectable markers for E. coli. The normal E. coli cells do not carry resistance against any of these antibiotics