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NEET
Exam
NEET
Subject
Zoology
Chapter
Biotechnology - Principles and Processes

NEET · Zoology · Biotechnology - Principles and Processes

What is the purpose of having recognition sites in a vector for...

What is the purpose of having recognition sites in a vector for gene cloning?

Options

  1. ATo ligate the vector to a foreign DNA
  2. BTo generate multiple fragments of DNA
  3. CTo eliminate nontransformants
  4. DTo visualize the separated DNA fragments

Correct answer

A. To ligate the vector to a foreign DNA

Step-by-step solution

In order to link the alien DNA, the vector needs to have very few, preferably single, recognition sites for the commonly used restriction enzymes. Presence of more than one recognition sites within the vector will generate several fragments, which will complicate the gene cloning. The ligation of alien DNA is carried out at a restriction site present in one of the two antibiotic resistance genes.

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