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What is the function of the origin of replication (ori) in gene cloning?

Options

  1. ATo control the copy number of the linked DNA
  2. BTo eliminate nontransformants
  3. CTo visualize the separated DNA fragments
  4. DTo ligate the vector to a foreign DNA

Correct answer

A. To control the copy number of the linked DNA

Step-by-step solution

This is a sequence from where replication starts and any piece of DNA when linked to this sequence can be made to replicate within the host cells. This sequence is also responsible for controlling the copy number of the linked DNA. So, if one wants to recover many copies of the target DNA it should be cloned in a vector whose origin support high copy number

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