NEET
- Exam
- NEET
- Subject
- Zoology
- Chapter
- Biotechnology - Principles and Processes
NEET · Zoology · Biotechnology - Principles and Processes
What is the role of selectable markers in gene cloning?
What is the role of selectable markers in gene cloning?
Options
- ATo select the transformants from non-recombinants
- BTo inactivate the foreign DNA
- CTo visualize the separated DNA fragments
- DTo ligate the vector to a foreign DNA
Correct answer
A. To select the transformants from non-recombinants
Step-by-step solution
In addition to ‘ori’, the vector requires a selectable marker, which helps in identifying and eliminating nontransformants and selectively permitting the growth of the >transformants. Transformation is a procedure through which a piece of DNA is introduced in a host bacterium (you will study the process in subsequent section). Normally, the genes encoding >resistance to antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin, etc., are considered useful selectable markers for E. coli. The normal E. coli cells do not carry resistance against any of these antibiotics