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What is the role of selectable markers in gene cloning?

Options

  1. ATo select the transformants from non-recombinants
  2. BTo inactivate the foreign DNA
  3. CTo visualize the separated DNA fragments
  4. DTo ligate the vector to a foreign DNA

Correct answer

A. To select the transformants from non-recombinants

Step-by-step solution

In addition to ‘ori’, the vector requires a selectable marker, which helps in identifying and eliminating nontransformants and selectively permitting the growth of the > transformants . Transformation is a procedure through which a piece of DNA is introduced in a host bacterium (you will study the process in subsequent section). Normally, the genes encoding >resistance to antibiotics such as ampicillin, chloramphenicol, tetracycline or kanamycin, etc., are considered useful selectable markers for E. coli. The norma

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