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What is the purpose of insertional inactivation of β-galactosidase gene in recombinant DNA cloning?

Options

  1. ATo produce blue color in the presence of a chromogenic substrate
  2. BTo differentiate recombinants from non-recombinants
  3. CTo simplify the selection of recombinants
  4. DTo eliminate nontransformants

Correct answer

B. To differentiate recombinants from non-recombinants

Step-by-step solution

Selection of recombinants due to inactivation of antibiotics is a cumbersome procedure because it requires simultaneous plating on two plates having different antibiotics. Therefore, alternative selectable markers have been developed which differentiate recombinants from non-recombinants on the basis of their ability to produce colour in the presence of a chromogenic substrate . In this, a recombinant DNA is inserted within the coding sequence of an enzyme, β-galactosidase. This results into inactivation of the gen

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