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What is the role of β-galactosidase gene in recombinant DNA cloning?

Options

  1. ATo produce blue color
  2. BTo differentiate recombinants
  3. CTo simplify selection
  4. DTo inactivate gene for synthesis of enzyme

Correct answer

D. To inactivate gene for synthesis of enzyme

Step-by-step solution

Selection of recombinants due to inactivation of antibiotics is a cumbersome procedure because it requires simultaneous plating on two plates having different antibiotics. Therefore, alternative selectable markers have been developed which differentiate recombinants from non-recombinants on the basis of their ability to produce colour in the presence of a chromogenic substrate. In this, a recombinant DNA is inserted within the coding sequence of an enzyme, β-galactosidase. This results into inactivation of the gene

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