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What is the purpose of using alternative selectable markers in recombinant DNA cloning?

Options

  1. ATo eliminate nontransformants
  2. BTo simplify the selection of recombinants
  3. CTo differentiate recombinants from non-recombinants
  4. DTo produce blue color in colonies

Correct answer

B. To simplify the selection of recombinants

Step-by-step solution

Selection of recombinants due to inactivation of antibiotics is a cumbersome procedure because it requires simultaneous plating on two plates having different antibiotics. Therefore , alternative selectable markers have been developed which differentiate recombinants from non-recombinants on the basis of their ability to produce colour in the presence of a chromogenic substrate. In this, a recombinant DNA is inserted within the coding sequence of an enzyme, β-galactosidase. This results into inactivation of the gen

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