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What is the significance of producing blue color in colonies during recombinant DNA cloning?

Options

  1. AIndication of insertional inactivation
  2. BPresence of insert
  3. CPresence of non-recombinant colonies
  4. DIndication of recombinant colonies

Correct answer

C. Presence of non-recombinant colonies

Step-by-step solution

Selection of recombinants due to inactivation of antibiotics is a cumbersome procedure because it requires simultaneous plating on two plates having different antibiotics. Therefore, alternative selectable markers have been developed which differentiate recombinants from non-recombinants on the basis of their ability to produce colour in the presence of a chromogenic substrate. In this, a recombinant DNA is inserted within the coding sequence of an enzyme, β-galactosidase. This results into inactivation of the gene

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