NEETZoologyBiotechnology - Principles and Processes
How is recombinant DNA introduced into host cells in the method of micro-injection?
Options
- ABy incubating the cells with recombinant DNA on ice
- BBy injecting DNA directly into the nucleus
- CBy bombarding cells with high velocity micro-particles
- DBy allowing a disarmed pathogen to infect the cell
Correct answer
B. By injecting DNA directly into the nucleus
Step-by-step solution
This is not the only way to introduce alien DNA into host cells. In a method known as micro-injection, recombinant DNA is directly injected into the nucleus of an animal cell . In another method, suitable for plants, cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA in a method known as biolistics or gene gun. And the last method uses ‘disarmed pathogen’ vectors, which when allowed to infect the cell, transfer the recombinant DNA into the host