NEET
- Exam
- NEET
- Subject
- Zoology
- Chapter
- Biotechnology - Principles and Processes
NEET · Zoology · Biotechnology - Principles and Processes
How is recombinant DNA introduced into host cells in the method of...
How is recombinant DNA introduced into host cells in the method of biolistics or gene gun?
Options
- ABy allowing a disarmed pathogen to infect the cell
- BBy injecting DNA directly into the nucleus
- CBy bombarding cells with high velocity micro-particles
- DBy incubating the cells with recombinant DNA on ice
Correct answer
C. By bombarding cells with high velocity micro-particles
Step-by-step solution
This is not the only way to introduce alien DNA into host cells. In a method known as micro-injection, recombinant DNA is directly injected into the nucleus of an animal cell. In another method, suitable for plants, cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA in a method known as biolistics or gene gun. And the last method uses ‘disarmed pathogen’ vectors, which when allowed to infect the cell, transfer the recombinant DNA into the host.