JEE Main202228 Jun 2022Morning ShiftChemistryGeneral Organic ChemistryActual
Which one of the following techniques is not used to spot components of a mixture separated on thin layer chromatographic plate?
Options
- AI 2 (Solid)
- BU.V. Light
- CVisualisation agent as a component of mobile phase
- DSpraying of an appropriate reagent
Correct answer
C. Visualisation agent as a component of mobile phase
Step-by-step solution
Thin Layer Chromatography is a technique used to isolate non-volatile mixtures. The experiment is conducted on a sheet of aluminium foil, plastic, or glass which is coated with a thin layer of adsorbent material. The material usually used is aluminium oxide, cellulose, or silica gel. The sample spots can be observed under a UV light chamber, I 2 solid or by spraying of an appropriate reagent which can react with the sample spot.