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NEET2013ZoologyBiotechnology - Principles and ProcessesActual

The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of

Options

  1. ANon-recombinant bacteria containing -galactosidase
  2. BInsertional inactivation of -galactosidase in non-recombinant bacteria
  3. CInsertional inactivation of -galactosidase in recombinant bacteria
  4. DInactivation of glycosidase enzyme in recombinant bacteria

Correct answer

C. Insertional inactivation of -galactosidase in recombinant bacteria

Step-by-step solution

The colonies of recombinant bacteria appear white in contrast to blue calories of non-recombinant bacteria because of insertional inactivation of alpha galactosidase in recombinant bacteria. Alpha galactosidase is a glycoside hydrolase enzyme that hydrolyse the terminal alpha galactosyl moieties from glycolipids and glycoprotein. It is encoded by the GLA gene. -galactosidase is an exaglycosidase, which hydrolyzes the -glycosidic bond formed between a galactose and its organic rhoiety.

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