NEET 2013
- Exam
- NEET
- Year
- 2013
- Subject
- Zoology
- Chapter
- Biotechnology - Principles and Processes
NEET 2013 · Zoology · Biotechnology - Principles and Processes
The colonies of recombinant bacteria appear white in contrast to...
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of
Options
- ANon-recombinant bacteria containing -galactosidase
- BInsertional inactivation of -galactosidase in non-recombinant bacteria
- CInsertional inactivation of -galactosidase in recombinant bacteria
- DInactivation of glycosidase enzyme in recombinant bacteria
Correct answer
C. Insertional inactivation of -galactosidase in recombinant bacteria
Step-by-step solution
The colonies of recombinant bacteria appear white in contrast to blue calories of non-recombinant bacteria because of insertional inactivation of alpha galactosidase in recombinant bacteria. Alpha galactosidase is a glycoside hydrolase enzyme that hydrolyse the terminal alpha galactosyl moieties from glycolipids and glycoprotein. It is encoded by the GLA gene. -galactosidase is an exaglycosidase, which hydrolyzes the -glycosidic bond formed between a galactose and its organic rhoiety.