JIPMER2012ZoologyBiotechnology - Principles and Processes
Gel electrophoresis is used for
Options
- Acutting of DNA into fragments
- Bseparation of DNA fragments according to their size
- Cconstruction of recombinant DNA by joining with cloning vectors
- Disolation of DNA molecule
Correct answer
B. separation of DNA fragments according to their size
Step-by-step solution
When genomic DNA extracted from any tissue of a plant or animal species is digested with a restriction enzyme, it is cleaved into segments. The segments of different sizes can be separated through gel electrophoresis. Gel electrophoresis involves movement of fragments or molecules on the basis if molecule size and shape under the influence of electric field. Endonucleases are enzymes that produce internal cuts called cleavage in DNA molecules. It cleaves DNA only within or near recognition sites, which have specifi