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The figure below shows three steps (I, II, III) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents?

Options

  1. AB-Denaturation at a temperature of about 98^ C separating the two DNA strands
  2. BA-Denaturation at a temperature of about 50^ C
  3. CC-Extension in the presence of heat stable DNA polymerase
  4. DA-Annealing with two sets of primers

Correct answer

C. C-Extension in the presence of heat stable DNA polymerase

Step-by-step solution

There are three steps in polymerase chain reaction namely. (i) Denaturation-at 94^ C for DNA strand separation. (ii) Annealing-binding of primer. (iii) Extension in presence of DNA polymerase which is heat stable.

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