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NEET2012ZoologyBiotechnology - Principles and ProcessesActual

Biolistics (gene-gun) is suitable for

Options

  1. Adisarming pathogen vectors
  2. Btransformation of plant cells
  3. Cconstructing recombinant DNA by joining with vectors
  4. DDNA fingerprinting

Correct answer

C. constructing recombinant DNA by joining with vectors

Step-by-step solution

In biolistic or gene gun method 1-2 g tungeston or gold particle (called microprojectile) coated with DNA to be used for transformation are accelerated to velocities which enable their entry into plant cells/nuclei. This technique has general applicability to plant species and can be used to deliver DNA into virtually all tissues.

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