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NEET 2012
Exam
NEET
Year
2012
Subject
Zoology
Chapter
Biotechnology - Principles and Processes

NEET 2012 · Zoology · Biotechnology - Principles and Processes

Biolistics (gene-gun) is suitable for

Biolistics (gene-gun) is suitable for

Options

  1. Adisarming pathogen vectors
  2. Btransformation of plant cells
  3. Cconstructing recombinant DNA by joining with vectors
  4. DDNA fingerprinting

Correct answer

C. constructing recombinant DNA by joining with vectors

Step-by-step solution

In biolistic or gene gun method 1-2 μg\mu \mathrm{g} tungeston or gold particle (called microprojectile) coated with DNA to be used for transformation are accelerated to velocities which enable their entry into plant cells/nuclei. This technique has general applicability to plant species and can be used to deliver DNA into virtually all tissues.
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