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What did Stanley Cohen and Herbert Boyer achieve in 1972?

Options

  1. AConstruction of an artificial plasmid
  2. BIsolation of the antibiotic resistance gene
  3. CLinking of the antibiotic resistance gene with a native plasmid of Salmonella typhimurium
  4. DDiscovery of restriction enzymes

Correct answer

C. Linking of the antibiotic resistance gene with a native plasmid of Salmonella typhimurium

Step-by-step solution

The construction of the first recombinant DNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid (autonomously replicating circular extra-chromosomal DNA) of Salmonella typhimurium . Stanley Cohen and Herbert Boyer accomplished this in 1972 by isolating the antibiotic resistance gene by cutting out a piece of DNA from a plasmid which was responsible for conferring antibiotic resistance.

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